2023
Haoui M, Vergara C, Lishko PV; Kir7.1 is the physiological target for hormones and steroids that regulate uteroplacental function. BioRxiv. 13 April 2023; doi: https://doi.org/10.1101/2023.04.11.536476.
2022
Tang S, Lu Y, Skinner WM, Sanyal M, Lishko PV, Ikawa M, Kim PS.; Human sperm TMEM95 binds eggs and facilitates membrane fusion. Proc Natl Acad Sci U S A. 4 October 2022; doi: https://doi.org/10.1073/pnas.2207805119; Epub 26 September 2022.
Skinner WM, Petersen NT, Unger B, Tang S, Tabarsi E, Lamm J, Halalian L, Smith J, Bertholet AM, Xu K, Kirichok Y, Lishko PV; Mitochondrial uncouplers impair human sperm motility without altering ATP content. BioRxiv. 8 August 2022; doi: https://doi.org/10.1101/2022.08.04.502752.
Goldberg E, Lishko PV, Papadopoulos V, Zirkin B.; Editorial: Endocrine and paracrine regulation of spermatogenesis. Front Endocrinol (Lausanne). 26 July 2022; doi: https://doi.org/10.3389/fendo.2022.984409.
Zhao Y, Wang H, Wiesehoefer C, Shah NB, Reetz E, Hwang JY, Huang X, Wang TE, Lishko PV, Davies KM, Wennemuth G, Nicastro D, Chung JJ.; 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nat Commun. 17 June 2022; doi: https://doi.org/10.1038/s41467-022-31050-8.
2021
Björkgren Ida, Chung Dong Hwa, Mendoza Sarah, Gabelev-Khasin Liliya, Petersen Natalie T., Modzelewski Andrew, He Lin, Lishko Polina V.; Alpha/Beta Hydrolase Domain-Containing Protein 2 Regulates the Rhythm of Follicular Maturation and Estrous Stages of the Female Reproductive Cycle. Frontiers in Cell and Developmental Biology 7 September 2021; doi: https://doi.org/10.3389/fcell.2021.710864
Ida Björkgren, Sarah Mendoza, Dong Hwa Chung, Monika Haoui, Natalie True Petersen, Polina V. Lishko; The epithelial potassium channel Kir7.1 is stimulated by progesterone. J Gen Physiol 4 October 2021; 153 (10): e202112924. doi: https://doi.org/10.1085/jgp.202112924
Haoui, Monika & Petersen, Natalie & Björkgren, Ida & Chung, Dong & Lishko, Polina. (2021). Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. 10.1016/bs.mie.2021.03.004.
Liu, B., Mundt, N., Miller, M., Clapham, D. E., Kirichok, Y., Lishko, P. V. Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells. J. Vis. Exp. (168), e62049, doi:10.3791/62049 (2021).
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2020
Gabelev Khasin Liliya, Della Rosa John, Petersen Natalie, Moeller Jacob, Kriegsfeld Lance J., Lishko Polina V. The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility. Frontiers in Cell and Developmental Biology, Volume 8, 2020, Page 426. doi.org/10.3389/fcell.2020.00426.
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Lenka Vyklicka, Polina V. Lishko. Dissecting the signaling pathways involved in the function of sperm flagellum. Current Opinion in Cell Biology, Volume 63, 2020, Pages 154-161. doi.org/10.1016/j.ceb.2020.01.015.
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2019
Skinner, W. M., Mannowetz, N., Lishko, P. V. and Roan, N. R. (2019). Single-cell Motility Analysis of Tethered Human Spermatozoa. Bio-protocol 9(5): e3182. DOI: 10.21769/BioProtoc.3182.
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Hwang et al., Dual Sensing of Physiologic pH and Calcium by EFCAB9 Regulates Sperm Motility, Cell(2019), doi: 10.1016/j.cell.2019.03.047
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2018
Melissa Miller, Samuel Kenny, Nadja Mannowetz, Steven Mansell, Michal Wojcik, Sarah Mendoza, Robert Zucker, Ke Xu, Polina V Lishko. Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation. Cell Reports. P2606-2613; doi: 10.1016/j.celrep.2018.08.016
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Nadine Mundt, Marc Spehr, Polina V Lishko. TRPV4 is the temperature-sensitive ion channel of human sperm. eLife. 268409; doi: 10.7554/eLife.35853.
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2017
Roan NR, Sandi-Monroy N, Kohgadai N, et al. Semen amyloids participate in spermatozoa selection and clearance. Schekman R, ed. eLife. 2017;6:e24888. doi:10.7554/eLife.24888.
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Mannowetz N., Miller M.R., and Lishko P. Regulation of the sperm calcium channel CatSper by steroids. Proc Natl Acad Sci USA, 2017 May 30;114(22):5743-5748. doi: 10.1073/pnas.1700367114.
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2016
Brown SG, Publicover SJ, Mansell SA, Lishko PV, Williams HL, Ramalingam M, Wilson SM, Barratt CL, Sutton KA, Da Silva SM. Depolarization of sperm membrane potential is a common feature of men with subfertility and is associated with low fertilization rate at IVF. Hum Reprod. 2016 Jun;31(6):1147-57. doi: 10.1093/humrep/dew056. Epub 2016 Apr 6. PMID: 27052499.
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Miller MR, Mannowetz N, Iavarone AT, Safavi R, Gracheva EO, Smith JF, Hill RZ, Bautista DM, Kirichok Y, Lishko PV. Unconventional endocannabinoid signaling governs sperm activation via the sex hormone progesterone. Science. 2016 Apr 29;352(6285):555-9. doi: 10.1126/science.aad6887. Epub 2016 Mar 17. PMID:26989199.
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Lishko PV. Contraception: Search for an Ideal Unisex Mechanism by Targeting Ion Channels. Trends in biochemical sciences. 2016;41(10):816-818. doi:10.1016/j.tibs.2016.08.002.
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Björkgren I, Lishko PV. Purinergic signaling in testes revealed. The Journal of General Physiology. 2016;148(3):207-211. doi:10.1085/jgp.201611676.
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2015
Sumigama S, Mansell S, Miller M, Lishko PV, Cherr GN, Meyers SA, Tollner T. Progesterone Accelerates the Completion of Sperm Capacitation and Activates CatSper Channel in Spermatozoa from the Rhesus Macaque. Biol Reprod. 2015 Dec;93(6):130. doi: 10.1095/biolreprod.115.129783. Epub 2015 Oct 21. PMID:26490839.
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Williams HL, Mansell S, Alasmari W, Brown SG, Wilson SM, Sutton KA, Miller MR, Lishko PV, Barratt CL, Publicover SJ, Martins da Silva S. Specific loss of CatSper function is sufficient to compromise fertilizing capacity of human spermatozoa. Hum Reprod. 2015 Dec;30(12):2737-46. doi: 10.1093/humrep/dev243. Epub 2015 Oct 8. PMID: 26453676.
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Syeda SS, Carlson EJ, Miller MR, Francis R, Clapham DE, Lishko PV, Hawkinson JE, Hook D, Georg GI. The Fungal Sexual Pheromone Sirenin Activates the Human CatSper Channel Complex. ACS Chem Biol. 2016 Feb19;11(2):452-9. doi: 10.1021/acschembio.5b00748. Epub 2015 Dec 16.
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Lishko P. and Kirichok Y. (2015). Signaling the differences between cilia eLife 4, e12760 10.7554/eLIFE.12760.
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2014
Mannowetz N., Naidoo N.M., Choo S.S., Smith J.F., and Lishko P.V. Slo1 is the principal potassium channel of human spermatozoa. eLIFE. 2013 doi:10.7554/eLife.01009; (Editor’s choice).
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Song R., Walentek P, Sponer N., Klimke A., Lee J.S., Dixon G., Harland R., Wan Y., Lishko P., Muriel L., Kessel M., and He L. miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110. Nature. 2014, Jun 4; 510(7503):115-20. doi: 10.1038/nature13413. NIHMSID 591060.
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2013
Lishko P., Clapham D.E., Navarro B., Kirichok Y. Sperm patch-clamp. Methods Enzymol. 2013; 525:59-83. PMCID: PMC3909741.
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Smith J.F, Syritsyna O., Fellous M., Serres C., Mannowetz N., Kirichok Y., and Lishko P.V. Disruption of the principal, progesterone-activated sperm Ca2+channel in a CatSper2-deficient infertile patient. Proc Natl Acad Sci USA. 2013 Apr 23; 110(17):6823-8. doi: 10.1073/pnas.1216588110. PMCID: PMC3637729.
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2012
Lishko PV, et al. The control of male fertility by spermatozoan ion channels. Annual review of physiology. 2012;74:453. doi: 10.1146/annurev-physiol-020911-153258.
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Ounjai P., Kim K., Lishko P. and Downing K.H. Three-Dimensional Structure of the Bovine Sperm Connecting Piece Revealed by Electron Cryotomography. Biol Reprod. 2012; 87(3):73; doi: 10.1095/biolreprod.112.101980.
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Fedorenko A., Lishko P.V. and Kirichok Y. The Mechanism of UCP1-dependent Thermogenic Uncoupling in Brown Fat Mitochondria. Cell. 2012 151(2): 400-13.
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2000 - 2011
Kirichok Y, Lishko PV. Rediscovering sperm ion channels with the patch-clamp technique. Molecular Human Reproduction. 2011;17:478–499. doi: 10.1093/molehr/gar044.
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Lishko PV, Botchkina IL, Kirichok Y. Progesterone activates the principal Ca2+ channel of human sperm. Nature. 2011 Mar 17;471(7338):387-91.
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Lishko PV, Kirichok Y. The role of hv1 and Catsper channels in sperm activation. J Physiol. 2010;588(Pt 23):4667–4672. doi: 10.1113/jphysiol.2010.194142.
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Lishko P.V., Botchkina IL, Fedorenko A, Kirichok Y. Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel. Cell.2010; 140; 327-337.
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Martemyanov K.A., Krispel C.M., Lishko P.V., Burns M.E., and Arshavsky V.Y. 2008. Functional comparison of RGS9 splice isoforms in a living cell. Proc. Natl. Acad. Sci. USA. 105:20988–20993 10.1073/pnas.0808941106.
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Phelps C.B., Procko E., Lishko P.V., Wang R.R., Gaudet R. Insights into the roles of conserved and divergent residues in the ankyrin repeats of TRPV ion channels. Channels. 2007;1:148–151. doi: 10.4161/chan.4716.
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Phelps CB, Huang RJ, Lishko PV, Wang RR, Gaudet R. Structural analyses of the ankyrin repeat domain of TRPV6 and related TRPV ion channels. Biochemistry. 2008;47:2476–2484. doi: 10.1021/bi702109w.
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Lishko PV, Procko E, Jin X, Phelps CB, Gaudet R. The ankyrin repeats of TRPV1 bind multiple ligands and modulate channel sensitivity. Neuron. 2007 Jun 21;54(6):905-18. PMID: 17582331.
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Strissel KJ, Lishko PV, Trieu LH, Kennedy MJ, Hurley JB, Arshavsky VY. Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. J Biol Chem. 2005;280:29250–5. doi: 10.1074/jbc.M501789200.
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Martemyanov K. A., Lishko P. V., Calero N., et al. The DEP domain determines subcellular targeting of the GTPase activating protein RGS9 in vivo. Journal of Neuroscience. 2003;23(32):10175–10181.
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Lishko P.V., Martemyanov K.A., Hopp J.A., Arshavsky V.Y. Specific binding of RGS9-Gbeta 5L to protein anchor in photoreceptor membranes greatly enhances its catalytic activity. J Biol Chem. 2002;277(27):24376–24381.
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Kondratskaya E. L., Lishko P. V., Chatterjee S. S., Krishtal O. (2002). BN52021, a platelet activating factor antagonist, is a selective blocker of glycine-gated chloride channel. Neurochem. Int. 40 647–653. 10.1016/S0197-0186(01)00109-7.
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1995-1999
Lishko, Polina V, et al. “Dependence of Pharmacological Activity of New NMDA Agonists and Antagonists on Their Chemical Structure .” Neurophysiology, vol. 31, no. 2, 1999, pp. 173–176., doi:10.1007/BF02515058.
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